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Stiffness and forces are two fundamental quantities essential to living cells and tissues. However, it has been a challenge to quantify both 3D traction forces and stiffness (or modulus) using the same probe in vivo. Here, we describe an approach that overcomes this challenge by creating a magnetic microrobot probe with controllable functionality. Biocompatible ferromagnetic cobalt-platinum microcrosses were fabricated, and each microcross (about 30 micrometers) was trapped inside an arginine–glycine–aspartic acid–conjugated stiff poly(ethylene glycol) (PEG) round microgel (about 50 micrometers) using a microfluidic device. The stiff magnetic microrobot was seeded inside a cell colony and acted as a stiffness probe by rigidly rotating in response to an oscillatory magnetic field. Then, brief episodes of ultraviolet light exposure were applied to dynamically photodegrade and soften the fluorescent nanoparticle–embedded PEG microgel, whose deformation and 3D traction forces were quantified. Using the microrobot probe, we show that malignant tumor–repopulating cell colonies altered their modulus but not traction forces in response to different 3D substrate elasticities. Stiffness and 3D traction forces were measured, and both normal and shear traction force oscillations were observed in zebrafish embryos from blastula to gastrula. Mouse embryos generated larger tensile and compressive traction force oscillations than shear traction force oscillations during blastocyst. The microrobot probe with controllable functionality via magnetic fields could potentially be useful for studying the mechanoregulation of cells, tissues, and embryos.

 

Abstract Understanding the human motor control strategy during physical interaction tasks is crucial for developing future robots for physical human–robot interaction (pHRI). In physical human–human interaction (pHHI), small interaction forces are known to convey their intent between the partners for effective motor communication. The aim of this work is to investigate what affects the human’s sensitivity to the externally applied interaction forces. The hypothesis is that one way the small interaction forces are sensed is through the movement of the arm and the resulting proprioceptive signals. A pHRI setup was used to provide small interaction forces to the hand of seated participants in one of four directions, while the participants were asked to identify the direction of the push while blindfolded. The result shows that participants’ ability to correctly report the direction of the interaction force was lower with low interaction force as well as with high muscle contraction. The sensitivity to the interaction force direction increased with the radial displacement of the participant’s hand from the initial position: the further they moved the more correct their responses were. It was also observed that the estimated stiffness of the arm varies with the level of muscle contraction and robot interaction force. 

Effective physical human-robot interaction (pHRI) depends on how humans can communicate their intentions for movement with others. While it is speculated that small interaction forces contain significant information to convey the specific movement intention of physical humanhuman interaction (pHHI), the underlying mechanism for humans to infer intention from such small forces is largely unknown. The hypothesis in this work is that the sensitivity to a small interaction force applied at the hand is affected by the movement of the arm that is affected by the arm stiffness. For this, a haptic robot was used to provide the endpoint interaction forces to the arm of seated human participants. They were asked to determine one of the four directions of the applied robot interaction force without visual feedback. Variations of levels of interaction force as well as arm muscle contraction were applied. The results imply that human’s ability to identify and respond to the correct direction of small interaction forces was lower when the alignment of human arm movement with respect to the force direction was higher. In addition, the sensitivity to the direction of the small interaction force was high when the arm stiffness was low. It is also speculated that humans lower their arm stiffness to be more sensitive to smaller interaction forces. These results will help develop human-like pHRI systems for various applications.